Hello,
Has anyone used the Roche HighPure viral RNA kit to extract RNA from cells? The idea for me is to use the same kit to extract RNA from supernatants of infected cells and from the cells themselves, in order to simplify the protocol.
Or if you know of another kit that is great for both cells and supes, let me know?
Thank you
@ Lionel Berthoux
Hi Lionel, I just recently came upon a protocol kindly made public by Fomsgaard AS & Rosenstierne MW (attached file) that elegantly bypasses the need to isolate Sars cov-2 viar RNA. Would that help if you are working in COVID-19?
Best. Alfonso
@ Lionel Berthoux
Alternatively, Sigma Aldrich makes an RNA isolation kit based in the Chomczynski P, Sacchi N protocol (Anal. Biochem. 162:156-159.1987). The original method was a real gem for isolating RNA and – for that reason even though I have not used this kit- I believe it could be a good substitute if fast RNA purification kits become scarce. A priori, I would think it could be used to isolate Sars cov2 RNA from both i) cell monolayers and ii) supernatants. However, the draw back is that it takes 4 hours.
Best. Alfonso
- Badges
- Science topic
- Similar topics
- Biological Science
- Molecular Biology
- Biomolecules
- Nucleic Acids
- RNA