Why would shorter transcripts take longer in a T7 in vitro transcription?

Lionel Uran Landaburu @Lionel-Uran-Landaburu-2

18 June 2020 0 2K Report

I was reading the T7 RNA polymerase protocol and noticed the following sentence regarding incubation time: "Incubate at 37°C for 1 hour. For shorter (< 300 nt) transcripts incubate at 37°C for 2–16 hours."

Can't think of a reason why short transcripts would take longer than long ones. Any ideas?

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