Dear all,
Can anyone suggest me a robust cAMP assay kit for Gi coupled GPCRs?
I would like to use this kit for cell lines stably expressing GPCRs that are coupled to Gi (inhibitory G proteins) where signalling leads to decrease in cAMP level.
I would be glad if you can share me the protocol (if different from the manufactures instruction) and any cautions.
Thank you so much !
With regards,
Parijat Sarkar
Hi Parijat,
Are you looking at ELISAs for this?
This could be a useful ELISA here:
https://www.elisagenie.com/product/camp-elisa-kit/
Seán
Hello,
We have used the LANCE cAMP 384 kit from PerkinElmer for Gi coupled GPCRs. It is a time-resolved FRET immuno assay.
https://www.perkinelmer.com/ch/lab-products-and-services/application-support-knowledgebase/lance/lance-camp-asay.html
or
http://www.perkinelmer.com/CMSResources/Images/44-73586MAN_LANCEcAMP384KitUser.pdf
Mariëlle
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