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Dear all, Can anyone suggest me a robust cAMP assay kit for Gi coupled GPCRs? I would like to use this kit for cell lines stably expressing GPCRs that are coupled to Gi (inhibitory G proteins)...
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Hello All, I am doing a MTT assay where because of drug treatment , the no. of cells in treatment is reducing to half of control. Now, after MTT assay how do i know the reduction in absorbance is...
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Without disturbing the flippases / floppases / ABC transporters, how can I break lipid asymmetry in live cell by chemical agent? Thanks !
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Hello everyone, Can actin polymerisation or depolymerisation regulate(up/down) lelvel of cholesterol in cell? The reverse relationship has been established well, but can actin cytoskeleton regulte...
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Can anybody suggest a software where I can specify one gene sequence, forward and reverse primer sequence and then the program will run a PCR with parameters set by me? All the available...
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