Hello all,

I am trying to isolate RNA from wheat grain samples at different developmental stages of grain filling duration and I successfully isolated RNA from one genotype but few samples from second genotype are creating a problem. The attached image shows RNA from different samples.The nanodrop readings are almost similar for all the samples but the gel image indicates only 5S RNA band in  7 of the 15 samples. So, is it the problem with the tissue itself or some problem with my extraction? Your suggestions will be appreciated.

Regards,

Gautam.

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