Helmi Y. Alfarra

6 Questions 25 Answers 0 Followers

Questions related from Helmi Y. Alfarra

Melting curve! What the factors that may affect on melting curve?

Hello every body. Using (SensiFast Sybr No-ROX one step kit) from Bioline, I did qPCR experiment used one -same- sample with 3 different concentrations of RNA and two different reference genes....

19 August 2016 3,576 6 View

Folate receptor Positive or Negative ?

Our lab have received cell lines panel. I would like to know which one in the panel is FR+ and which one is FR- ? Can any one hint me please? Thanks

08 June 2016 2,380 3 View

Is it acceptable to do wounds healing experiments on cancer cell line?

I have met some one told me that he did some experiments on wounds healing using cancer cell line instead of normal cell line. I it applicable to do that and to publish the results?

13 November 2013 9,921 9 View

How can I check, if the isolated compound is novel or already known?

Is there any quick method to know, if the isolated phytochemical is novel or already known? The structure of the compound was already identified using NMR spectra and EI-MS spectra.

07 September 2013 5,997 11 View

Confused about results of thin layer chromatography (TLC).

I have these results of TLC, I am not sure why this happens or whether the products are the same or not? Please see the picture attached bellow. This TLC was stained by vanilin and this is the...

04 February 2013 7,268 19 View

How to check if crystals are organic or inorganic compounds?

I have some crystals with different shapes and some are cube like. I have run a methanolic extract of Centella asiatica in a silica column and one of the fractions, after a long time, showed some...

31 January 2013 2,177 48 View