I'm doing FACS and some of the sorted populations are smaller than 500cells. I would like to extract TOTAL RNA for RNA-seq and I was thinking of sorting straight into lysis buffer to avoid centrifugation following the sort. I don't know though if there is any kit (or protocol) that would be suitable for real-total-RNA extraction from so few cells. (I know trizol is not recommended) Any suggestions?
Jack M Gallup
Your idea of sorting directly into the lysis buffer is a good idea (to avoid sample loss); also the attached file may be of some use to you.
0 votes
0 thanks
- Badges
- Science method
More Chrysanthi Taxiarchi's questions See All
Similar questions and discussions