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Questions related from Chrysanthi Taxiarchi
I am FAC sorting testicular cells and I would like to do FISH to tell the spermatogenic stage. Some populations are very rare ~1,000 cells, so I wanted to sort them straight onto slides and do the...
17 March 2017 2,951 3 View
I'm doing FACS and some of the sorted populations are smaller than 500cells. I would like to extract TOTAL RNA for RNA-seq and I was thinking of sorting straight into lysis buffer to avoid...
20 August 2016 8,908 1 View
I would like to isolate total RNA from single cells for qPCR (or even sequencing later on). What protocol or kit should I use to obtain good quality RNA with minimum loss?? I've seen there is a...
14 April 2016 8,915 3 View
I am not familiar at all with FACS but I would like to use it to isolate cells from a tissue and then do RNAseq of the sorted cell populations. These cells are expressing either only RFP...
27 April 2015 4,018 4 View
