Autocorellation function - how is compute existance of hbond in GROMACS?

GROMACS version: 2018.4 GROMACS modification: No I have a question. I want to know one thing. How is computing autocorrelation function, because I am not sure about something. I try to calculate...

07 February 2021 9,525 1 View

What are the limits of determining metastability in the brain?

What are temporal limits in determining brain metastability? In other words, how long does the minimum length of the scan segment using fMRI have to be for the result to be of any value? I'm...

07 February 2021 2,380 2 View

Ansys Fluent- Velocity Inlet/ Pressure Outlet/ Symmetry/ Wall- how are the BC formulated from the viewpoint of mathematics?

Hello, I went through the Fluent Theory Guide and other Ansys learning resources, however I couldn't find the answers to the following issues. Let's discuss the problem on the example of a...

01 January 2021 1,686 3 View

Does Fluent/CFX use Reynolds-averaging when the viscous model is set to Laminar? What about icoFoam?

Hello, this question is not as trivial as it may seem. The Laminar model in Fluent/CFX is pretty stable even when the physics are not laminar at all (internal flow, Re=20k, bifurcations/pipe...

23 December 2020 4,848 3 View

How to measure volume per lipid in Gromacs?

I have a question. I want to measure the volume per lipid. I built a membrane with water and run the simulation for 300 ns. To measure the volume per lipid I used a .gro file from the simulation...

22 October 2020 2,282 3 View

Can I use repeated measures ANOVA for within-litter design developmental experiments?

I am planning a developmental experiment where I will treat neonatal mice and then I will perform test after they reach adulthood. I plan to use within-litter design as follows: I will use 12...

07 October 2020 8,539 13 View

How to compute the order parameter for the last carbon in lipid tail in membrane GROMACS?

01 August 2020 5,756 2 View

SAFT90 soccer match simulation protocol - how can i find audio record?

I am interested in simulating a soccer match using the SAFT90 protocol. While reading the papers that have used it, I understood that an audio track is required. Can anyone address me to where I...

20 March 2020 759 2 View

How can I learn parametrization by myself in molecular modelling?

Is it possible to learn parametrization by myself? Do you know some good books, videos about that? I found that https://www.ks.uiuc.edu/Training/Tutorials/science/ffTK/fftk-tutorial.pdf but I...

01 March 2020 8,688 4 View

How to apply DAPC (adegenet) on Geometry morphometry data?

Do you have some suggestions about how to apply DAPC (adegenet R package) analysis on the Geometry Morphometry dataset? The dataset contains 12 morphometric points characterized by XY...

09 February 2020 1,690 2 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Do you know good and user-friendly programs to measure plant quantitative traits?

For my research i will need to measure plant quantitative traits (especially leaves area and roots length, but would be nice to add some more information). I recently discovered...

01 March 2021 5,035 2 View