Hao Wu

5 Questions 11 Answers 0 Followers

Questions related from Hao Wu

What are these endogenous autofluoresence particles in maize leaf protoplasts (see picture below)?

I used reference wild-type maize (W22 and B73) leaf protoplasts without any fluorescence protein introduced. But I saw some odd autofluorescence particles in some (not all) protoplasts, some cells...

22 June 2017 1,572 5 View

How to combine two RNA-seq normalized data sets from different sources for WGCNA?

I am new in WGCNA. I have two RNA-seq data sets collected from two different developmental stages of maize endosperm (developmental stages can be considered as "interesting" condition). Both data...

05 January 2017 8,725 5 View

How can I deal with the secondary structure in primers with restriction site?

I have a problem on primers with restriction site. Here are my primers Forward_XmaI: TCTATA(CCCGGG)GACC.... (34 mer, GC=52.9%) Reverse_SalI: AACACT(GTCGAC)TTACA...(32 mer, GC=43.8%) The template...

07 June 2015 1,793 9 View

What is the annealing temperature of the T7 promoter primer or terminator primer?

I am using the T7 promoter primer or terminator primer with a gene specific primer to test my ligation before transformation, but the the Tm value of T7 primers is about 10C lower than my gene...

10 April 2014 2,871 1 View

How to reduce the degradation of GST-tagged protein?

Hi, I am trying to express a GST-tagged protein using E.coli BL21(DE3) competent cells. I think this protein seems to be degraded even in the cells, because even I doubled the proteases inhibitor...

01 January 1970 5,530 14 View