Could let know the quantity of rhizobias (are in liquid) to inoculate in to the 100g soil for determining nitrogen fixation?
You need the rhizobium spore count between 108and 1010 CFU/mL and at least 15 to 20 mL , depends on your soil quality to mix your 100 g of soil .
Each
isolate of PGPR was grown overnight in Kings’ B
broth. Each broth was centrifuged at 12,000 rpm,
washed with phosphate buffer three times and then
pellets were dissolved in 0.1M phosphate buffer
(pH 7.0) and OD was adjusted to 1.2 before being
used for pot experiments. An OD of 1.2 was
equivalent to 2.0108 cfu ml1. The seeds for each
treatment were soaked for an hour in phosphate
saline buffer (PSB) containing the suspension of the
PGPR isolates to maintain a population of approximately
108 cfu seed1. In each pot, eight seeds (95%
germination) were sown at a depth of 5 cm. After
germination, five seedlings were maintained in
each pot.
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