Results on Parameter-Dependent Pareto Front?

Dear community, I would like to request some references related to results on parameter-dependent Pareto Front, if there are any. I am interested in studying the behavior of the Pareto Front with...

08 May 2019 9,669 3 View

How do I quantify cells and fibers in tissues used in immunohistochemistry?

Hi all, I would need to compare the number of cells and fibers in different part of a tissue which was used in fluorescent immunohistochemistry. However, I am not sure how to proceed. I could use...

21 March 2019 8,043 3 View

What Big Data solution use for deep learning on clinical data purpose ?

Hello everyone, I would like to perform machine learning and deep learning in a near futur on clinical data, coming from different sources and technologies (Omics data, Imaging data, and so...

23 August 2017 3,551 5 View

NLP : Short text classification/categorization

Hello everyones, I'm looking for a way to classify really short pieces of text (max 18 words) into 9 different classes. As a learning set, I've got a dataframe with each sentence (one column)...

12 July 2017 2,965 10 View

Change of metric and uniform continuity?

Dear all, we know, owing to a result appearing in the book of R. M. Dudley (Real analysis and Probability - Theorem 2.8.2), that for any separable metric space (S, d), there is a metric e on S,...

19 April 2017 4,159 6 View

Weak convergence of signed measures?

Dear all, I am searching a result concerning the weak convergence of signed measures defined on the Borel algebra of a given metric space. The result that i find so far is established for weak...

10 February 2017 2,604 3 View

Relation between $\mu$-continuity sets and the Borel sigma-algebra?

Dear all, For any sigma-finite measure $\mu$ on the Borel subsets of a metric space X (separable if needed), we recall that a $\mu$-continuity set is any Borel set B such that $\mu(boundary of B)...

23 January 2017 3,301 4 View

What is the dual space of bounded continuous functions defined on a metric space (not necessarily compact)?

What is the dual space of bounded continuous functions defined on a metric space (not necessarily compact)? (i would appreciate to have a reference for the result too)

16 January 2017 6,534 8 View

Has anyone noticed high or uneven fluorescence background with iBlot PVDF membrane stacks?

I was wondering if anyone else has used the iBlot PVDF membrane stacks and noticed uneven and/or relatively high autofluorescence / background when used for fluorescence Western blotting? I am...

29 May 2016 8,048 2 View

ELISA from HUVEC cell culture supernatant

I want to do an ELISA on HUVEC cell culture supernatant to check for TNF alpha release. Can anybody recommend a suitable cell density/ml medium? I am thinking to culture my cells in a 6 well...

17 February 2016 5,274 2 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

Difficulty in cloning of 1kb gene into vector?

I am facing difficulties in cloning a 1kb gene into a vector (pJIT163). I have my gene on interest (GOI) in pUC57 and want to clone in pJIT163 using SalI and BamHI restriction sites. I am getting...

25 February 2021 3,221 7 View

Is it harmful to breed for Cre Homozygosity initially?

Hello, I am currently using a Cas9 knock in system that will be expressed in the liver. To do so STOP codon fl/fl Cas9 KI mice were bred with Albumin-Cre mice to generate heterozgous pups, that...

24 February 2021 5,759 3 View

Are there lab markers/pens resistant to Roti-Histol?

Hi, I'm looking for a pen/marker to write on the smooth, transparent (non-frosted) part of microscopy slides which does not get washed away by alcohols and most importantly Roti-Histol. I want to...

24 February 2021 9,902 5 View

I have a problem with kanamycin - why the kanamycin doesn't affect?

Hi everyone, I need to expression of GFP with pET28 plasmid in E. coli BL21(DE3) plysE. Transformed cells have kanamycin and chloramphenicol resistance. Normally, only transformed cells should...

22 February 2021 4,032 4 View

How does elongation affect the resistance of an electrode?

What are the testing rate for electrodes recommended by ASTM for cochlear implants. I am currently doing the 15mm/min elongatio testing.

22 February 2021 1,515 1 View

Resistivity of PVD TiNx thin films vs. N2 flow?

In the PVD of TiN thin films (~100A), I've found the resistivity of the TiN films dropped from ~100 to 60um*cm with N2 flow increasing up to the metallic mode ending point; then the resistivity...

21 February 2021 1,864 2 View

Where can the fault diagnosis data set of lithium battery be found?

Most of the data sets on the network only contain good information about the voltage and internal resistance of lithium batteries. The data of faulty battery is missing. Who can help me, I will be...

21 February 2021 8,659 1 View

Alternatives to traditional cloning of insert into backbone (dsDNA)?

Hi, I am stuck with a cloning experiment: The insert & backbone(bb) share one compatible end at 3' end(NotI) and one incompatible end at 5' end (XbaI for insert; HindIII for backbone). So i...

16 February 2021 1,664 3 View