Resistant capacity of Microcystis in lake

More Nathalie Nouchet's questions See All

What is the best device to measure artificial light at night ?

Hello, I would like to know if someone could advice me about the best device to use to measure artificial light at night in coastal areas, outside and inside water (just few meters)? Thank you...

23 April 2024 8,319 1 View

Anyone doing research on Leveraging AI for Equitable Performance Management/Appraisal practices?

I am pursuing my PhD and chose the topic of pros/cons and ethical concerns in leveraging Artificial Intelligence tools to increase Equity in Performance Management/Appraisal and did not find much...

18 November 2023 8,460 2 View

How to calculate ID/IG ratio of carbon materials measured by Raman?

I want to calculate the ID/IG ratio from a carbon sample measured by Raman. After obtaining the spectra, I have deconvoluted D and G peaks by fitting them with 2 Gaussian-Lorentzian curves using...

16 November 2023 6,458 1 View

Hello Can I put a html file (a network graph) ?

08 June 2023 613 1 View

What is a good percentage of organic carbon in a coffee crop?

I want to know how much organic carbon is a good percentage in a coffee crop, having in consideration that it will be taken from the first 30 centimeters of the sampling point.

14 May 2023 7,638 2 View

What post hoc tests used after a fisher exact test (2 x 3) in R ?

I have contingency table with 2 treatments (t1, t2) and 3 stages (s1, s2, s3) as following: T1 T2 s1 x1 x2 s2 y1 y2 s3 z1 z2 I got a significant interaction when i did...

18 January 2023 8,942 4 View

Which analyses suits best for moderation?

Currently I'm struggling with choosing between various analysis options, ranging from repeated measures design, to 4-way ANOVA to ANCOVA or to moderation analysis with the PROCESS macro of Hayes....

17 January 2023 1,628 4 View

Shannon and Bray-Curtis indices for presence/absence data. Not appropriate, wrong or not ideal but ok... ?

Hi, I need to compare the alpha diversity of three communities (the gut bacteria; the gastro-intestinal helminth community ; the bacteria community found in the spleen of rodents) For the gut...

29 September 2022 4,851 2 View

Circular data in SPSS?

I have some polar transformed data that I would like to represent in a graph. I was used to do that in Statistica; however, I cannot find a way to do it in SPSS. Does someone know if/how to do it...

28 March 2022 5,436 3 View

Conducting Q methodology research online - Does anyone have any practical recommendation or tip?

For one of my studies, I am using Q methodology for the first time. I am currently developing the Q-survey that will be administered to participants on an online platform (I am considering using...

14 March 2021 8,837 3 View

Is it common for heterotrophic bacteria to grow on Pro99 and SNAX media?

I am attempting to isolate picocyanobacteria from seawater (pre-filtered with a GF/D membrane) using the pour plate technique and filter plating method (Kearney et al., 2022) on Pro99 and SNAX...

30 July 2024 1,040 1 View

What is the best way to drop a plasmid?

Please address the best way to drop a plasmid. Background: I have a "bait" plasmid resistant to kanamycin and a "prey" plasmid resistant to carbenicillin. After many rounds of streaking on...

25 July 2024 2,532 3 View

Pink bacterial colonies?

I am cloning an overexpression plasmid with my protein of interest tagged with mScarlet. After transforming my ligated product into DH5α bacteria and plating on LB agar, I noticed colonies with a...

22 July 2024 5,953 6 View

Cell proliferation assays suggestions?

What cell proliferation assay do you suggest? cyQUANT from thermo Fischer has good reviews, a lot of good articles for the kit from Abcam. and Promega kits look easy and straight forward.

21 July 2024 1,554 2 View

Method of collection of samples of microorganisms from environmets to labortary?

can any one guid me about tractical method for preservation of microorganism same as bacteria, cyanobacteria and algea, in sampling from environment to Laboratory: 1- Sample collection 2-...

17 July 2024 3,687 11 View

My rosetta strain has chloramp resistance and i am transforming my plasmid with amp resistance but not getting colonies on chloramp/amp LB plates?

I have done the same procedure with BL21 and pLysE but i got colonies but multiple times on rosetta i did but not getting. can anyone explain what would have gone wrong?

12 July 2024 9,904 1 View

Has anyone calculated the effective concentration at 50% (EC50) value of different fungicides for the fungus Tilletia horrida?

How can we measure their colony diameter?

12 July 2024 7,027 0 View

I have constucted a plasmid vector from puc19 with no antibiotic and lacz gene. The problem is screening for transformed cells. Any suggestions?

Get too many colonies, which are mostly non-transformants when tested by colony PCR

11 July 2024 6,775 3 View

I am not getting proper colonies while ligating one of fragments in my vector the colonies seems to be normal but in light it seems lysed any reason?

I am working on cloning of a gene fragment inside my vector everything we tried to do correctly like making insert and vector with utmost care but after ligation the morphology of colonies seems...

11 July 2024 9,010 3 View

Quelles applications innovantes peuvent bénéficier des propriétés des géopolymère?

Les géopolymères offrent plusieurs propriétés intéressantes qui peuvent être exploitées dans diverses applications innovantes : 1. Construction durable : - Bétons à faible empreinte carbone -...

10 July 2024 7,457 1 View