Dear All,
I am growing rice plants in the greenhouse. These plants were inoculated with arbuscular mycorrhizal community with 500 spores/kg dry soil. The rice plant is 45 days old. I took rice root samples for checking the infection of arbuscular mycorrhiza in the root stained by trypan blue 0,01%. When i checked the infection of arbuscular mycorrhiza in the rice root, I recognized these infected forms which was not as I was expected. I would like to know who they were in the pictures. Could you please help me to identify the name of the spores inside the rice root. Thanks you very much in advance.
Yours sincerely,
Xuan Do Thi
Xuan Do, the root coloration allows you to appreciate the establishment of the symbiosis that has taken place between the roots of the rice plant and the arbuscular mycorrhizal fungus. So for the identification of the spores of each strain of fungi present, it would be better to evaluate these spores in the soil substrate used by the method of Gerdemann and Nicholson (1963) or of Daniels and Skipper (1983) or S. Shamini, 2016 or S. Shamini and K. Amutha, 2014 for example
Kindly check https://link.springer.com/article/10.1007/s40003-019-00404-y
Also check https://www.tandfonline.com/doi/full/10.1080/00380768.2016.1277156
Dear Xuan Do, I don't think you have any AMF colonization in the roots on your photo. But it is not entirely sure what you are asking. Perhaps the dark spots are spores of some fungal endophyte, but not of AMF.
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