15 November 2022 0 9K Report

I am looking for non-renewable energy alternatives for renewable energy indexes, namely ECO, ERIX, SPG, and SUN.Any suggestions are highly appreciated.

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Does anyone know, can i do cryosectioning of whole aorta after oil red O staining and use the cryosection parts for further analysis?

I would like to quantify the lesions on aorta. I want to take image of atherosclerotic plaques formation in aorta by using O red Oil Solution. After the staining, what will be the storage...

16 February 2021 5,876 2 View

What is the best concentration of Pam3CSK4 to coat a dish to activate with THP-1?

Hi everyone I am trying to coat a glass-bottom dish with Pam3CSK4 and plate THP-1 cells to measure IL6 with ELISA from the supernatant. I wondered if anyone knows a good concentration and...

21 January 2021 2,593 1 View

Why I see Lamp-1 GFP in cytocol after transfection?

Hi every one I transfected Lamp-1 GFP plasmid to the HEK293 cell line. When I image the cells in TIRF, I see green fluorescence o the cytosol too. Does anyone have any idea that how it can...

21 December 2020 9,978 3 View

Why HEK293 transfected with LAMP-1GFP do not load dextran?

Hi every one I have made a cell line of HEK293 transfected LAPM-1GFP and after seeding the cells for 24h, I try to load dextran on the dish but after imaging, the cells with GFP+ have loaded less...

14 December 2020 1,801 2 View

What are the tools or software which can simulate climate change for a city and conclude future climate data?

I am working in research on climate adaptation so need to compare the performance of the urban fabric to the climate changes, which requires me to make a comparison and simulation of the...

03 December 2020 988 8 View

Correlation, Regression and significance?

Dears, I have different variables in multiple regression. Two variables are highly correlated. To solve the collinearity issue, I dropped one of them from the multiple regression model. The...

29 November 2020 2,695 4 View

Do anyone have any authentic material regarding Cultural Ethics and especially regarding Pakistani culture?

I need this because I have to represent it in a conference as a keynote speaker

17 November 2020 584 4 View

How can I synthesis heparin nano particle?

I want to synthesize iron nanoparticles with heparin, but I have trouble synthesizing First of all, do I have to use a freezer drying for drying of product and it is not impossible use from...

16 November 2020 9,455 2 View

How to differentiate between relationship and effect using statistical terms?

It is easy to understand the meaning of relationship and effect (impact) in general. But when it comes to the statistical analysis it is a difficult conundrum, as one can determine the...

24 July 2020 6,525 7 View

Which is the best solvent for herbal extraction?

We are aware of the fact that some type of solvent is required for isolating a compound from herbs. I personally believe that water is the best solvent since mostly decoction is prepared using...

22 July 2020 4,179 2 View

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After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

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Should we change media after1st 6hrs after plating HUVEC bought from INVITROGEN?it's not recomended by invitrogen.Will DMSO present in stock matter?

We have HUVEC bought from Invitrogen. Cat no -C-003-5c. while first plating the cells we have bought the protocol suggests not to centrifuge the cell with media to remove the DMSO. Should we...

02 March 2021 3,713 2 View

Is SSRN e-Journal Scopus indexed?

I have publication in SSRN. I want to know whether it is Scopus indexed? Does it show impact factor? What is its ISSN No.?

02 March 2021 4,565 3 View

Any research paper call for publication related to management or finance or economics?

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01 March 2021 8,379 1 View

How to find fast publication Scopus Indexed (Q2) journals in Computer Science and Engineering?

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Scopus Index Journal List , 2021?

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27 February 2021 3,545 1 View

How to calculate Ligand-active site distance in a ligand-protein complex from MD simulation?

I have run a MD simulation of a ligand-protein complex in GROMACS using CHARMM forcefield. Reviewer has asked me to calculate Ligand-activesite distance and binding free energy of ligand with...

27 February 2021 4,703 1 View

How can be researcher's index developed?

What are the criteria to be followed to develop the researcher's index?

26 February 2021 9,051 0 View

The Shannon's index is inversely proportional to Simpson's index (1-D)?

I'm having a problem with my bloxplot with Shannon's and Simpson's indexs. The value of Shannon for my Community 1 indicating higher diversity (in relation of the Community 2), but have lower...

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