I have two different adherent cell lines currently seeded on gels. I want to remove the adherent cells without disturbing the ECM. What would you recommend I use in order to make this possible? Thanks!
You can try a trypsin-free solution like Accutase if you want viable cells, or, if you are just interested in the ECM, you could lyse the cells with ammonium hydroxide Article A Rapid, Scalable Method for Isolation, Functional Study and...