What pH buffer solution best replicates physiological conditions?

I am studying the interaction between protein (HSA) and liposomes (made with DMPC) and I would like to study under physiological pH for application in studying protein interaction with DDS. I have...

10 November 2020 3,594 3 View

How to interpret multilevel model with categorical variables (fitted using brm() in R)?

I am using brm() in R to fit a multilevel model for repeated measures data. The two predictors are both categorical. One is `Task` with two levels (`LO` and `ID`), another one is `Condition` with...

10 November 2020 712 4 View

Can Scheirer Ray Hare test be used in unbalanced data and sample size <5?

Hi everyone, I have a "tricky" dataset with two factors but is unbalanced and sample size small than 5. And this data is not a normal distribution but a bimodal. So I think ANOVA is not very...

29 July 2020 2,707 1 View

All teacher educators are under the lockdown since March, what could they be doing, how is this lockdown going to affect tutors' performance ?

There are many COVID 19 related issues in terms of how to deal with the continuum of teaching and learning. I am meditating about what the future performance results will be for both the student...

16 June 2020 199 3 View

Has anyone ever done research on employment for people with disabilities especially in public service?

I am currently doing my research on employment rate of people with disability in public service. I would be glad if someone has done or is doing the same topic.

19 March 2020 4,631 3 View

How to statistically analyze bad replication experiment data ?

11 March 2020 7,545 7 View

What is wrong with the plates?

I conducted a plaque assay in a 24-well plate. All 12 of the plates had the same problem shown in the attached pictures. I did a mock assay before this and it went well without any problems. Does...

25 December 2019 3,833 2 View

Ways to avoid dystocia of transgenic mice?

I am trying to breed Rag-/- mice and I found high percentage of female dead by dystocia. Is there any way to avoid this issue?

10 September 2019 3,668 1 View

What is an acceptable sampling rate for skin conductance?

What is an acceptable sampling rate for measuring skin conductance? Research seems to use various rates, from 32Hz to 1000Hz. Is there a minimum rate advised? I have a device that measures at 4Hz...

03 February 2019 9,630 1 View

How to measuring extracellular ATP level?

How to measuring extracellular ATP level in tumor specimens, not the culture cell? Is there are commecial Kits available?

17 November 2018 1,053 1 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

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03 March 2021 5,360 2 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

What is the characteristic length to determine the Reynolds number for the scattered turbulent fluid flow?

Dear all, I just saw this example in FB on the difference between laminar flow and turbulent flow. The flow from a pipe hits the sink and scattered. The flow from the pipe is characterized as...

02 March 2021 1,999 5 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

What is the most reliable source to get Impact factor of journals?

Source to get Impact factor of journals

01 March 2021 6,654 2 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View