What are the samples that shows positive confirmation for ninhydrin test?

Is we have any raw sample extract that show positive confirmation in ninhydrin test?

01 February 2019 7,705 5 View

What are the applications of thermodynamics in biological sciences? Is it possible to connect with some natural metabolic process with examples ?

What are the application of thermodynamics (Physical Chemistry) in Biotechnology ?

07 August 2018 2,941 9 View

What were the job opportunities one should get after acheiving a PhD Degree in Biotechnology both private and government presently in india?

Job opportunities after perceiving a Doctorate degree in biotechnology in India presently?

02 March 2018 699 3 View

Generally in what problematic situations in plants does a molecular phylogeny study should be proceeded?

What were the general inferences concluded after the phylogeny reconstruction?

10 November 2017 7,661 2 View

Is it possible to select a phylogenetic tree by finding the bootstrap value in mega software?

Bootstrap value X mega software

09 October 2017 8,310 2 View

Which phylogeny software will give a single tree with 2 or 3 different combination of plastid gene sequences?

Say for example a combination of MATK and RBCL gene in plants ?

09 October 2017 1,363 3 View

Which software can align both forward and reverse DNA sequences(Direct PCR Product Sequences) and fill gaps for phylogenetic analysis?

How can we get a single sequence from two separate forward and reverse sequenced PCR product ? Is it possible by bioedit software?

08 September 2017 5,568 2 View

What were the basic steps and softwares used in a phylogenetic analysis ?

Can anyone explain it with an example and steps followed?

08 September 2017 1,503 4 View

How horizontal gene transfer between tree species in dioecious plant occurs naturally ?

06 July 2017 9,023 3 View

What are the elements in a particular plant of different species from different phytogeography found in different type of sexual systems?

06 July 2017 1,787 1 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Which fluorescent dye to use for measuring total ROS production?

Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?

01 March 2021 9,447 3 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

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Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View