How can we get a single sequence from two separate forward and reverse sequenced PCR product ? Is it possible by bioedit software?
Yes. Regarding a doc that you can get from the first address:
1- Select the Reverse fasta form file name from the left-hand side and press Shift+Ctrl+R to generate a reverse complement strand. Now the forward and reverse sequences are running in the same direction and have (mostly) the same nucleotides.
2- Double-click on the file name to the left of the sequence to open a new editing window.
3 - Highlight and copy the entire sequence (Ctrl+C)
4- Go to the Forward sequence fasta window. Select “new sequence” under the “Sequence” feature in the top toolbar.
5- Paste your Reverse sequence in the new window (Ctrl+V)
6- Rename this new sequence “R” in the “Name” field.
7- Select “DNA” for “Sequence Type” to get the appropriate nucleotide colors.
8- Select “Apply and Close.” Now both sequences should show up in your Forward window.
For more information about how to fill gaps by analyzing chromatograms, I suggest you follow the already asked question in the second address.
https://discover.mbl.edu/downloads/Using_Bioedit.doc
https://www.researchgate.net/post/How_to_generate_consensus_DNA_sequence_contig_from_forward_and_reverse_sequence_Which_software_will_I_use
Genious or codon code aligner can do it, however, they are not free.
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