Hello
What will be the optimum sample no of MTB in WGS using Miseq V3x600
Any one is using MYKROBE for analysis of WGS data .I want to know about interpretation
13 September 2020 2,642 2 View
Please let me know the thermocylin parameter for amplification of pncA gene in MTB using following primer set if any one use this pncA_F3 (AAGGCCGCGATGACACCTCT) and pncA_R4...
25 June 2019 9,185 3 View
06 March 2016 7,888 5 View
nMOLDYN has the capability to use the velocity auto correlation function to obtain the phonon density of state for an NVT simulation. There are problems with normalization of the spectra. Does...
10 March 2014 9,647 3 View
Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...
25 February 2021 6,969 3 View
Hello, I have demultiplexed my short reads and I have removed the Illumina adapter left in the sequences and now I need to remove the barcodes used by the sequencing centre from my reads. I have...
22 February 2021 9,873 3 View
Hi, I am working on some 16S sequencing data, and seems some of them are low quality. I am not sure how to set the trunc value in dada2. qiime dada2 denoise-paired \ --i-demultiplexed-seqs...
30 January 2021 6,213 3 View
I got my fungi sequences back from Illumina Miseq sequencing and I'm wondering how many reads do you use as a cutoff? ie. do you delete anything with less than 100 reads? I can't seem to find any...
28 January 2021 5,646 4 View
Genotyping data is from Illumina Global screening array 24 v 2.0 and ConsistencyDupSNP.sh file contains list of pair of duplicated SNPs in GSA24 v 2.0.
18 December 2020 1,966 3 View
I am interested in the different tools that can be used to create custom databases for targeted sequencing and how to trim the databases based on the amplicon size? Also, should custom databases...
02 December 2020 3,105 3 View
I am looking to run illumina miseq data through dada2, but the limited identification of this gene means there are no pre-build databases. I have a database built in MOTHUR format, does anyone...
30 November 2020 8,228 3 View
I downloaded the expression matrix data "GSE143416_Brain_data_TPM.txt.gz" of GSE143416 from Gene expression omnibus ( https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi). However, the first column of...
30 November 2020 1,304 3 View
Adding heterogeneity spacers to primers used for Illumina based amplicon studies has proven to improve read quality by increasing bp diversity on the flow cell during sequencing. I have seen two...
01 November 2020 9,071 3 View
I am looking for opinions/recommendations of NGS Platforms (Illumina vs. BGI/MGI) from people that had opportunity to work on both hardware: MGISEQ-T7 and NovaSeq 6000 or analyze data from both....
04 October 2020 8,125 1 View