Does anyone recommend any publication on the Japanese concept of "mabusabi" - specially refering to Shinohara Motoaki's works?

I am looking for materials that study Shinohara's concept of mabusabi and its demonstration in literature and visual arts.

10 November 2020 6,580 1 View

Recent bibliography on Jesuits' dictionaries and "artes"?

Hello, colleagues, Greetings from Brazil. I would like to know if you could recommend recently published material (open access, preferably) addressing Jesuits' dictionaries and "artes" from 16th,...

21 October 2020 1,046 1 View

What are the existing open bite classifications?

I need updated information on existing open bite classifications. If they are based on cephalometric measurements even better.

11 January 2020 6,846 1 View

Input requirements of Isle Optics TS100 acousto-optic modulator?

In my lab I have an old AOM from Isle Optics Ltd, model TS100. I don't know what kind of signals it requires, and looking around for similar models by other companies, I guessed I needed a...

20 November 2019 5,986 2 View

Antibodies to Multiple Marking in Confocal Microscopy?

31 March 2019 5,041 3 View

Best projection for calculating 3D surfaces in ArcGIS?

Dear all, currently I am calculating surface area in ArcGIS (forests in Ethiopia) and am noticing the differences between the projections I am trying out. I am working with equal area projections...

10 March 2019 8,460 6 View

Does anybody knows if exist a triple trasgenic model of Alzheimer's disease from Jackson Laboratory (JAX) that has a c57bl6 background?

I have to use an animal model of Alzheimer's disease that shows beta amyloid plaques and also Tau hyperphosphorylation but the only model that I found has a 129 background and I need a model...

08 April 2018 4,623 3 View

I'm making a data mining project, but I need a lot of sample of emails containing malicious pdf attachments. Where can I find that sources?

I need to know were can I find this kind of sources for my data mining research project. Thanks in advance

09 April 2017 5,309 2 View

PCoA 3d plotting using python3?

Hello, I have performed a PCoA analysis in GenAlEx using microsat data and obtained the 2d plot. Since there is no way of making it 3d in GenAlEx, I'd like to use either the raw distance matrix or...

22 November 2016 2,966 3 View

RIETVELD REFINEMENT (Fullprof): What causes chi square to be smaller than one?

Last week I ask about the goodness of the fit of XRD with Fullprof. I received than many suggestions about the refinement process. Unfortunately, I still become a chi sqare smaller than one (what...

14 September 2016 9,056 4 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

How vaccines of covid 19 work?

Millions ask how vaccines of covid 19 be successful and safe, its here to explain generally how vaccine of pfizer work ,mRNA vaccines teach our cells how to make a protein that will trigger an...

28 February 2021 2,723 4 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Best plasmid could be used as a standard curve for AAV titration?

I have been trying to have a good AAV titration for my production and I have tried different plasmids to set up as a standard curve but each one behaves differently. i am trying to use ITR for...

23 February 2021 5,167 3 View

How can I stain the somatodendritic part of inhibitory neurons in a hippocampal culture with high specificity?

I would like to distinguish the somatodendritic part of the inhibitory neurons in a hippocampal culture (where we expect to have 5-10% of GABAergic neurons). What are the best antibodies and...

23 February 2021 6,756 1 View

How to control the variants vof covid 19 in second and third waves?

A the world find means to control covid 19 by vaccination ,new variants came to spread and make the world in fear and danger, to understand the conduct of these variants, es constantly change...

21 February 2021 3,080 6 View

Surprisingly low antibody titers against SASRS-COV-2 RBD in hamsters compared to mice?

21 February 2021 6,958 2 View

The PC12 cell line characteristics?

Hello dear scientists, Does the undifferentiated PC12 cell line in terms of chromosome characteristics, proliferation, and drug reactions have the characteristics of cancer cells? Where is it...

21 February 2021 8,945 3 View

Will SARS-CoV-2 Spike be broken into S1 and S2 by denaturing conditions?

Hello, I am performing a western blot of my SARS-CoV-2 Spike-pseudotyped lentiviral particles. I denature my virus by adding concentrated virus to 2X Laemmli buffer at 1:1, then boiling for...

17 February 2021 4,844 4 View

How to calculate the TCID50 when the highest CPE is 50% or less?

I am doing TCID50 to measure progeny virus and some treatments protected the cells in a way that the highest CPE observed is 50% or less. How should I do the titre calculation in that case? Thank you!

16 February 2021 8,768 1 View