RedEXTRACT-N-Amp PCR ready mix--can it be used without the kit?

08 February 2023 0 2K Report

Hello, can the RedEXTRACT-N-Amp PCR ready mix be used without the kit? I have DNA from tissues digested with other digestion methods. Can I use this for my PCRs?

Thank you,

Badges
Science method

More Sreemoyee Acharya's questions See All

Please suggest a good text book for "Research Methodology in Life Sciences". Which book would be good for PhD Scholars?

Please provide Book Title and author name

02 March 2021 9,059 3 View

Why are the two terms -research methods/research methodology - used interchangeably?

Two terms - research methodology and research methods - in research methodology books are clearly differentiated. Yet, these terms are loosely used in many research articles; particularly in the...

13 December 2020 6,147 7 View

Is folic acid cytotoxic to human kidney cells ?

My group is interested in folic acid cytotoxicity in human kidney cells. Are there any reports available showing toxic effects of FOLIC ACID as in the case of mice kidneys? Any insights will be...

19 October 2020 2,273 3 View

Has anyone done magnetic target localization using Genetic Algorithm?

I am trying to model magnetic target localization in MATLAB using those algorithms. So essentially what I want to output is the position vector of the target (x, y, z) and the magnetic moment...

28 August 2020 9,811 2 View

What tests can i run to see if the distributions of the training set and test set data are similar?

I have two datasets from different sources: One is training and the other is the Test dataset. I extract features from the training set images and train the model. The trained model is then used...

16 June 2020 7,737 1 View

Would you please be kind to share the possible ways to quantify the amount of mucus in the airway in the picture attached below?

I tried imageJ but ImageJ threshold option could not isolate the color of the airway only.

20 May 2020 8,792 2 View

What might be the reason for High OD of background in TNF alpha ELISA?

My protocol of ELISA is Capture Antibody coating (4 micrograms per ml) incubation overnight. Blocking (1% BSA in PBS) incubation for 1 hour. Sample loading (100 microliters of BAL fluid diluted in...

22 April 2020 3,577 3 View

How to interpret the results obtained from X-ray diffraction (XRD)?

I prepared the gold nanoparticles using plant extract and characterized using XRD. Result displays four prominent diffraction peaks indexed as (111), (200), (220) and (311) miller indices which...

28 June 2019 7,767 10 View

Which software use for making cif file of different materials???

i m doing work on semi conductor alloys i can not find a cif files of some materials..pls help me out.

29 April 2019 5,857 3 View

Can ALE be applied to composite structure in Abaqus CAE. if yes then how can we do it ?

it shows an error 1.adaptive meshing is not supported in any elements in this domain. 2.adaptive meshing contraints can be specified on adaptive mesh domain.

10 March 2019 6,460 1 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Research on Supply Chain Management in Indian context?

Hi, I am planning to apply for the PhD degree in the Supply Chain Mgt. with specific area of "Cold Storage warehouses" during Pandemics and wars. Where lock downs and shut downs are frequent....

02 March 2021 285 2 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

Anyone that can help with details in phosphopeptides enrichment?

Anyone know the amount of protein before enzymatic digestion for phosphopeptides enrichment of serume sample in Ti-IMAC? Has anyone ever use Ti-IMAC(the material is from Chinese company named...

28 February 2021 7,310 3 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

Can thermal cycler machines be problematic during PCR?

Hi, my question is about the heating of thermal cycler machines and I hope some of you had experienced a similar thing previously. There are two thermal cycler machines in the lab(BioRad) and for...

26 February 2021 4,777 4 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...

24 February 2021 6,310 3 View

Is this the write procedure for acid digestion of soil for heavy metal analysis using AAS?

How to do acid digestion of soil sample with 3:1 aquaregia in an open vessel?

23 February 2021 3,053 7 View