Recommended Ca2+ concentration for optimal MGL activity?

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Can we mark 'EFL Learners shifting from general digital to AI technologies' as technological transition?

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Vanessa Porkolab

Hi !

I characterized (during my phD thesis) different C-type lectins including MGL with their carbohydrate-bases binders using surface plasmon resonance (SPR). I will recommend you to work with this type of buffer: 20 mM Tris pH 7, 150 mM NaCl, 4 mM CaCl2. In this buffer, you should observe nice protein activity and binding.

Vanessa

Andreia Peixoto

Thank you very much Vanessa!