Andreia Peixoto

4 Questions 3 Answers 0 Followers

Questions related from Andreia Peixoto

Why do cells resist antibiotic selection but fail to overexpress the desired protein signatures?

Dear all, I have tried to use a mammalian Expression Vector System to overexpress a protein of interest in AGS cell line. Cells were selected based on puromycin resistance conferred by successful...

19 August 2024 5,823 2 View

How to extract protein from human dendritic cell cultures?

I have proceeded to extract whole protein from human monocyte derived dendritic cells, using regular lysis with RIPA lysis buffer followed by centrifugation to obtain soluble protein suspension...

11 September 2023 8,169 1 View

How can I design an experiment capable of demonstrating if my cells can grow without a substrate?

Dear Researchers, I am incredibly confused about the in vitro colony forming assay. I was asked to perform such assay to assess my cell models capability to grow without attachment to a solid...

11 June 2020 6,368 3 View

Recommended Ca2+ concentration for optimal MGL activity?

Hi everyone, I would like to start some experiments using the macrophage galactose-type C-type lectin (MGL) and I’m trying to find some recommendations on the Ca2+ concentrations to use to obtain...

09 January 2020 3,288 2 View