Currently working on the nutritional profile of the seed proteins of Hyptis spicigera as a global public good, I am looking for the following analytical services:

1. extraction the total seed proteins;

2. separation of the constituent proteins by sequential precipitation under a range of pH buffers, as described by Aguirre et al. (2012)* for the related species H. suavolens;

3. purification of the constituent proteins, with a buffer change to non-Tris (e.g. PBS) buffers, whereupon the amino acid profile of each constituent protein can be determined by AQC derivatization.

Thanks,

Eliot

* ‘Analysis of Protein Fractions and Some Minerals Present in Chan (Hyptis suaveolens L.) Seeds’. Journal of Food Science 71(1): C15-C19.