Currently working with colleagues in northern Uganda on the nutritional profile of the seed proteins of Hyptis spicigera as a global public good, I am seeking analytical collaboration (and co-publication) in this technical area.
With some limited funding, our team is also actively seeking referrals to a reliable and competent contract laboratory, offering this analysis on a fee-for-service basis.
Specifically, we are looking to
1. extract the total seed proteins;
2. separate the constituent proteins by sequential precipitation under a range of pH buffers, as described by Aguirre et al. (2012)* for the related species H. suavolens;
3. purify the constituent proteins, with a buffer change to non-Tris (e.g. PBS) buffers, whereupon
4. the amino acid profile of each constituent protein can be determined by AQC (6-aminoquinolyl-N-hydroxysuccinimidyl carbamate) derivatization, by a current project partner.
Thanks for your attention, and thanks in advance for any interest or referrals.
Please contact me directly at Eliot.Masters (at) nmit.ac.nz or sheanut (at) gmail
* ‘Analysis of Protein Fractions and Some Minerals Present in Chan (Hyptis suaveolens L.) Seeds’. Journal of Food Science 71(1): C15-C19.
Hi Eliot, good day
you could try to contact Eurofins lab team. https://www.eurofins.com/
Maybe they have interest in participate the project.
my best wishes.
Isaac
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