Hi everyone,
We are synthesizing a virucidal material that will kill SARS-CoV-2 on contact.
In these preliminary stages, we want to use enveloped surrogate viruses that are the easiest to propagate and safe to handle. For example, Phi6 bacteriophage and its easily cultured host, Pseudomonas syringae.
Other CoVs (299e, OC43, etc.) and influenzas have been considered, but as I said for now we want to keep complications to a minimum.
Any advice on possible enveloped viruses to use?
Thanks,
Max Bueckert
Just a couple thoughts.
Sindbis virus. BSL2, has readily available infectious clones that make it easy to acquire and propagate, is a human virus, and it forms very nice plaques/there are infectious clones for high throughput assays (gfp/luciferase expressing).
Tomato spotted wilt virus is an enveloped plant virus. Much harder to culture and assay but more safe.
Chloroviruses. Also harder to culture and more safe.
Dear all, as a polymer researcher I would like to propose two ways to reproduce the preparation of clones viruses ones:
- template polymerization.
https://www.researchgate.net/publication/229855092_Template_Polymerization
DOI: 10.1039/C4PY01522C
- Molecularly imprinted polymers
https://doi.org/10.1016/j.febslet.2006.04.037
https://doi.org/10.1002/anie.201805772
My Regards
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