Can I use the CHARMM GUI input files for Gromacs in HPC?

Good day everyone! I am a Biochemistry undergraduate in the Philippines and to be very honest, we have no background in bioinformatics since we really focused on wet lab techniques + theory. Due...

15 February 2021 3,422 4 View

Cause of slow bromophenol blue migration and smearing while running SDS PAGE?

While running gels for western blotting, I am currently getting, what seems to be, the blue dye from my sample buffer migrating more slowly in sample lanes leaving blue streaks. These transfer to...

08 February 2021 5,317 3 View

Same sample, significantly different trace for DMF vs. THF for cyclic voltammetry?

Dear reader, I've been having trouble with my cyclic voltammetry recently, but I then had a break through and managed to get apparently "clean" results using dry, deoxygenated THF and/or DMF,...

06 January 2021 4,312 3 View

Can I use GROMACS for field-theoretic simulation of copolymers?

I need to know if I can use GROMACS instead of LAMMPS for my undergraduate thesis wherein I will generate phase diagrams of copolymers using FTS. Thank you so much!

22 November 2020 3,725 3 View

How to quantify lncRNA/miRNA in ribosomal depleted RNA-seq?

I did some ribosomally depleted RNA-seq in rat brain. Does anyone have recommendations on how to quantify/compare small RNA expression between samples? I have used RNA STAR to map reads back to...

11 November 2020 5,331 1 View

What are some essential steps when using THF for cyclic voltammetry?

I'm new to electrochem/cyclic voltammetry. I'm interested in measuring cyclic voltammograms of organic semiconductors in organic solvents, specifically, THF. I know that in principle I should be...

09 November 2020 2,298 4 View

At what stage of development do animals develop sentient?

I'm trying to find out in the development between being an embryo and an adult animal does the animal become phenomenally conscious? I'm especially looking for recommendations for articles on the...

30 August 2020 3,815 3 View

How to depolarize cultured primary neurons with KCl without them dying?

14 August 2020 1,488 8 View

Recommendations on model enveloped viruses that are easy and safe to propagate?

Hi everyone, We are synthesizing a virucidal material that will kill SARS-CoV-2 on contact. In these preliminary stages, we want to use enveloped surrogate viruses that are the easiest to...

08 August 2020 6,731 4 View

Where can I purchase fluorescent silica nanoparticles?

Hi all, I want to purchase fluorescent silica nanoparticles (with and without a functional group to make it hydrophobic) in powder form. A variety of size options would be key. Preferably, if you...

04 August 2020 7,440 6 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

I got 02 moderator values defining Johnson-Neyman significance regions. Is it an error?

Hi everyone, I am conducting research for my Master's thesis. I am using PROCESS by Johnson-Neyman to analyze my Moderator model. I test the relationship between Public Service Motivation and...

03 March 2021 2,350 2 View

What analysis to choose with a large neste dataset and clearly skewed or kurtotic distributions?

Hello! I have a dataset of n=3000 nested within 8 countries with approximately 200 or 400 responses in each country. I originally planned to perform multilevel modelling with 4 dependent variables...

02 March 2021 6,865 1 View

Do you know any MR image dataset?

I would like to research on MR images (0.5T and 3T). Can you please suggest some websites that I can download dataset including both 0.5T and 3T MR images? Thank you.

02 March 2021 7,735 3 View

Can NFL theorem be valid in infinite search space in ML?

NFL theorem is valid for algorithms training in fixed training set. However, the general characteristic of algorithms in expanded or open dataset has not been proved yet. Could you show your...

01 March 2021 1,189 3 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

No title

01 March 2021 3,905 5 View

What test should I use to compare two or three means, if data is not normally distributed but sample size is > 30 in each group?

I personally feel this as a most confusing question. As per my understanding, if sample size is > 30, and sample is skewed, we can still use paramteric approach, as it will follow Z...

01 March 2021 1,587 6 View

Does anyone have kinetic and kinematic dataset for gait classification?

I want to differentiate between normal and abnormal movement. For this reason, I am looking forward to this type of dataset.

01 March 2021 9,485 1 View

Is it necessary to report effect size (ES) in statistics of biomedical research given that we already report p values routinely?

Pls share your thoughts

01 March 2021 7,330 4 View