We have a project using MC65 cells which are a Tet-OFF line for expression of the C99 fragment of APP. Tetracycline withdrawal produces cell death in this line within 2-4 days.
We want to look at ABeta toxicity but ideally want to look at effects from the full length APP protein instead of the C99 fragment.
Does anyone know if there is a stable transfected line out there with full length APP over-expression that can be used for similar toxicity measures?
Aderemi Caleb Aladeokin
Hi,
You might want to consider using the Mouse Neuro2a (N2a) neuroblastoma cells stably overexpressing the human Swedish mutant (K670N, M671L) APP (N2aAPPsw, clone Swe.10). This is available as a gift (I think) from Dr G. Thinakaran (The University of Chicago, IL, USA).
Good luck.
Aderemi.
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