Andrew Michael Pickering

4 Questions 2 Answers 0 Followers

Questions related from Andrew Michael Pickering

Where can I buy Nitrate mineral salts medium (NMS)?

I am trying to get my hands on Nitrate mineral salts medium (NMS). The only source I could find is ATCC® MD-1306 which has discontinued this product . Does anyone know of another source?

19 December 2018 6,239 2 View

Recommendations on a good stable transfected APP line for cytotoxcity assays?

We have a project using MC65 cells which are a Tet-OFF line for expression of the C99 fragment of APP. Tetracycline withdrawal produces cell death in this line within 2-4 days. We want to look...

20 December 2017 7,834 1 View

Can you do Neomycin selection in a transfected cell line which already has a neomycin resistance construct inserted?

I want to transfect an already established transfected cell line with a construct of mine. The issue is that the cell line has been previously modified with a construct carrying a neomycin...

18 December 2017 1,765 4 View

Can Streptomycin mimic Tetracycline?

I have been having trouble getting a published TET-OFF cell culture line to work. I have been finding that the treated and not-treated cells look pretty much the same. It occurred to me that...

30 November 2017 3,206 5 View