How to determine the significance of "diagonal" contrasts in two-way ANOVA interactions on SPSS?

Hi all, I'm running a 2 x 2 between-subjects ANOVA with the setup in the attached image (kindly refer to JPG attachment). I understand that calculating simple effects involves determining...

09 February 2021 547 3 View

Are there any alternatives to trasnfection efficiency?

We'd like to test our nanoparticles for transfection efficiency, however, due to COVID 19, limitations hinder us from doing so. Are there any cheap alternatives? Preferrably in plants or other...

06 September 2020 6,398 3 View

What are the other tests which can measure transfection efficiency using nanoparticles without the involvement of cells? Preferably cheap. ?

What are the other tests which can measure transfection efficiency using nanoparticles without the involvement of cells? Preferably cheap. Please we need an answer asap. Other than the buffering...

27 January 2020 765 3 View

What bacterial cells can I use (preferably cheaper with good transfection efficiency) aside from DH5 Alpha cells from E. coli for gene delivery?

I am planning on conducting a research on gene delivery, however , we only have a limited amount of sources, can you suggest bacterial cells that I can use (preferably cheaper with good...

28 August 2019 5,667 3 View

What exactly is the difference between using 95% ethanol and methanol in making a Bradford reagent?And is the filtering process necessary?

I've seen 95% ethanol and methanol being mentioned in protocols and I was wondering whether using one over the other have any drastic affects on the reagent (ie longevity, quality, color...

14 August 2019 292 3 View

What strain of mice should I use for a study in Toxoplasma cyst reduction?

19 July 2017 5,407 1 View

Is there a questionnaire for teachers to assess the use of the five basic elements of cooperative learning?

I'm looking for a questionnaire for teachers to assess the use of the five basic elements of cooperative learning in the classroom (elementary school). It's for my masterthesis. Thankyou!

19 January 2017 5,932 4 View

Can Anyone Advise on Running Omnibus Test of Group Differences in SmartPLS?

Read 4 answers by scientists to the question asked by Gabrielle Daniels-Gombert on Jul 12, 2016

11 July 2016 5,941 3 View

Which applications do you consider important for the health area?

Which applications do you consider important for the health area?

01 January 1970 3,273 11 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

Would fecal samples stored at -20C for over a year impact metagenomic analysis?

Dear All, I have access to some rodent fecal samples that have been stored at -20C instead of -80C. Anyone have experience with the impact of stowage condition on comparative metagenomic analysis...

01 March 2021 2,512 1 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View