Recommendation for Saliva Exosome Isolation Kit

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Is there a subtle distinction between the terms "substitution" and "doping"?

Dear Prof, is there a subtle distinction between the terms "substitution" and "doping" when referring to modifying ceramic dielectric materials for example Na0.5Bi0.5TiO3? Is there any difference...

08 July 2024 2,043 3 View

Problems/ Challenges regarding Dark Tourism?

Im currently writing my Thesis on Challenges/ Problems/ Particularities regarding Dark Tourism and have a little trouble finding the right literature. I have found a lot of literature for dark...

14 June 2024 7,262 2 View

When IC50 cannot be obtained according to the cell viability chart, what should be the dose selection under cell culture conditions?

When IC50 cannot be obtained according to the cell viability chart, what should be the dose selection under cell culture conditions? Would it be beneficial to go with sham control? For example,...

11 June 2024 9,109 0 View

How to calculate my FRAP Assay?

Y=0.0011x+0.1031 (Standard curve eq) Chamomile sample 20mg/mL Absorbance(y)= 1.16 x=960.82(µg/mL) Chamomile sample 10mg/mL Absorbance(y)= 0.80 x=633.55(µg/mL) Tridax sample...

01 June 2024 7,843 0 View

What are the long-term functional and aesthetic outcomes of autologous vs alloplastic materials in facial reconstructive surgery following trauma?

This question aims to explore the comparative effectiveness of using a patient's own tissue (autologous) versus synthetic materials (alloplastic) in facial reconstructive procedures, focusing on...

25 May 2024 1,791 0 View

How to determine the concentration of MXene colloidal solution?

Dear researchers, I have recently synthesised MXene by etching MAX phase source using in-situ fluoride based etchant and collected the supernatant solution obtained after the washing process. My...

22 May 2024 5,269 2 View

Do your country have Psychology Acts?

Hi/Salam; Did your country establish "Psychology Acts" for psychologists to practice? I just discovered that Malaysia still does not have the Psychology Acts for psychologists to practice, where...

13 May 2024 6,921 6 View

Dear Prof and Dr, how to calculate the recoverable energy density from P-E hysterisis loop ?

The recoverable energy density can be evaluated by the following equation: Wrec = ∫P EdP = Area CDBCJcm−3 (4) Thus, when domains are realigned by the applied electric field, the difference...

08 May 2024 4,561 0 View

Hi, does anyone has example Silvaco code text for Graphene field effect transistor?

I would like to learn the details coding GFET through SILVACO but need reference. Hoping anyone could help me

06 May 2024 7,681 1 View

RMSD plot from GROMACS output file .xtc did not start from 0ns. How to get the 0ns frame?

Dear GROMACS expert, I am currently having trouble to analyse the results from my simulations. After processing the output files using this command, gmx trjconv -s md_0_1.tpr -f md_0_1.xtc -o...

02 May 2024 6,690 7 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

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Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

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Is it common for heterotrophic bacteria to grow on Pro99 and SNAX media?

I am attempting to isolate picocyanobacteria from seawater (pre-filtered with a GF/D membrane) using the pour plate technique and filter plating method (Kearney et al., 2022) on Pro99 and SNAX...

30 July 2024 1,040 1 View

Question about MHB (Mycorrhiza Helper Bacteria) isolation technique??

What are the most effective isolation techniques for obtaining MHB?? Is there are best approach techniques? I keep struggling in obtaining it.

26 July 2024 7,387 0 View

Question about Single Strain Arbuscular Mycorrhiza Fungi??

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26 July 2024 6,520 1 View

I found the zeta potential of exosomes in DLS and it was -8mv. So is it correct or not ?

What should be the zeta potential of bovine milk exosomes

25 July 2024 7,376 5 View

Isolation of PLV6-Bmal-Luc plasmid?

I am currently at a stage in my research where I will be using the pLV6-Bmal-luc plasmid (Addgene #: 68833). However, I am encountering some issues with the isolation of this plasmid and am unable...

23 July 2024 5,296 0 View

Can I use sonicator only to lyse the exosomes and measure their protein contents using BCA assay kit?

Hello, I want to ask regarding exosome protein quantification method. Is it necessary to lyse exosomes before measuring their protein contents using BCA assay kit? Or can I just straight away...

23 July 2024 4,603 4 View

The best source for amplification of ADAM17 prodomain?

hi every one I am making vector construction (for fusion proteins) and in this moment I wanna to amplification of ADAM17 prodomain with PCR. to yet, I couldn't amplified the ADAM17 prodomain with...

21 July 2024 8,660 1 View

Alexander Sasha Vlassov

Total exosome isolation reagent (Invitrogen)- there are several of them: cell culture media, serum, plasma, "other body fluids" with a protocol specifically for saliva, including low sample input

Nur Basirah Ghazali

Thank you Dr Alexander Sasha Vlassov

Bhairavi Vajaria

http://www.101bio.com/files/manual_P530.pdf