How to remove unedited version of the manuscripts from research gate?

Our manuscript was published ahead of print. The edited and revised version is now available. How do I remove the old version of the manuscript from the research gate?

01 February 2021 5,965 1 View

What causes up shift of protein band in Western Blot?

Hello, I am working on a recombinant protein expressed in the form of inclusion bodies for my bachelor thesis. After solubilization and refolding I performed an SDS-PAGE and to confirm the...

21 October 2020 5,311 8 View

How to prevent false negative FISH results?

I am analyzing data from a FISH experiment and notice that there are false negatives. What is the best option to change in the experimental set up to avoid this from happening?

04 October 2020 5,777 1 View

Can you suggest an academic platform (in addition to RG and Academia) where PhD students can share drafts of their papers for feedback?

I am looking for a platform where PhD students would feel comfortable to share their papers at a very early stage of research and could receive a helpful feedback.

05 August 2020 4,713 3 View

Difficulty in interpreting data processed with two-way ANOVA?

I have a two-factor experiment. We investigated the effect of the drug on the level of erythrocytes after surgery. One of the factors is the presence of the operation, the second is the presence...

22 July 2020 7,687 5 View

Do you have information about the available free program for determining the area of leaves using a photo or image scanner?

21 June 2020 2,297 5 View

Please, help. Should one or two-way ANOVA be used in case of determining the drug effect of the drug against the background of the model operation?

We studied the effect of a biological supplement on biochemical and hematological parameters rabbits with and without model operation. I have 4 group of rabbits (n=8) and 3 time points (before...

15 May 2020 5,846 17 View

Does anyone know of a free biology software NOT SUBJECT TO COPYRIGHT for publishing papers?

Does anyone know of a free biology software NOT SUBJECT TO COPYRIGHT for publishing papers?

24 February 2020 7,748 3 View

How to analyse measurements from 3 different conditions?

I have cytokine measurements from 3 different conditions: What is the best way of comparing the differences between the conditions ? Many thanks Irina

20 February 2020 5,750 3 View

Possible problems with eNOS intracellular staining in circulating endothelial cells?

I am trying to set up a protocol to study circulating endothelial cells by flow cytometry. First approach was to identify CD45-CD146+ population and analyze expression of cell adhesion molecules...

28 January 2020 9,190 3 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

How to avoid ulcers in s.c. injected tumors ?

Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...

01 March 2021 9,938 3 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View

Benefits of the 24 welled plate?

We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...

28 February 2021 1,149 1 View