How to remove unedited version of the manuscripts from research gate?

Our manuscript was published ahead of print. The edited and revised version is now available. How do I remove the old version of the manuscript from the research gate?

01 February 2021 5,965 1 View

What causes up shift of protein band in Western Blot?

Hello, I am working on a recombinant protein expressed in the form of inclusion bodies for my bachelor thesis. After solubilization and refolding I performed an SDS-PAGE and to confirm the...

21 October 2020 5,311 8 View

How to prevent false negative FISH results?

I am analyzing data from a FISH experiment and notice that there are false negatives. What is the best option to change in the experimental set up to avoid this from happening?

04 October 2020 5,777 1 View

Can you suggest an academic platform (in addition to RG and Academia) where PhD students can share drafts of their papers for feedback?

I am looking for a platform where PhD students would feel comfortable to share their papers at a very early stage of research and could receive a helpful feedback.

05 August 2020 4,713 3 View

Difficulty in interpreting data processed with two-way ANOVA?

I have a two-factor experiment. We investigated the effect of the drug on the level of erythrocytes after surgery. One of the factors is the presence of the operation, the second is the presence...

22 July 2020 7,687 5 View

Do you have information about the available free program for determining the area of leaves using a photo or image scanner?

21 June 2020 2,297 5 View

Please, help. Should one or two-way ANOVA be used in case of determining the drug effect of the drug against the background of the model operation?

We studied the effect of a biological supplement on biochemical and hematological parameters rabbits with and without model operation. I have 4 group of rabbits (n=8) and 3 time points (before...

15 May 2020 5,846 17 View

Does anyone know of a free biology software NOT SUBJECT TO COPYRIGHT for publishing papers?

Does anyone know of a free biology software NOT SUBJECT TO COPYRIGHT for publishing papers?

24 February 2020 7,748 3 View

How to analyse measurements from 3 different conditions?

I have cytokine measurements from 3 different conditions: What is the best way of comparing the differences between the conditions ? Many thanks Irina

20 February 2020 5,750 3 View

Possible problems with eNOS intracellular staining in circulating endothelial cells?

I am trying to set up a protocol to study circulating endothelial cells by flow cytometry. First approach was to identify CD45-CD146+ population and analyze expression of cell adhesion molecules...

28 January 2020 9,190 3 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

What are the main reasons to grow Escherichia coli O157:H7 and Salmonella Typhimurium in food products under refrigeration storage 4 C?

What are the main reasons to grow Escherichia coli O157:H7 and Salmonella Typhimurium in food products under refrigeration storage 4 C?

24 January 2021 9,987 3 View

I am trying to simulate phase change of refrigerant inside pipe extracting heat from panel in comsol. Can anyone help with how to simulate this?

My model is a rectangular multi layered structure under which refrigerant flows through cylindrical pipes. A heat flux of 600W/m2 applied on panel and internal forced convection is used for...

20 January 2021 9,478 1 View

Is it possible to calculate entalphy of LiBr/H2O or another solution if we know correlation of specific heat without using any software?

Is it possible to calculate entalphy of LiBr/H2O or another solution if we know correlation of specific heat without using any software?

17 January 2021 5,522 3 View

How do i calculate the total heat transfer rate in COMSOL (5.4)?

I have modelled a condenser of a refrigerator in COMSOL. I made different designs and i want to calculate which design has the highest heat transfer rate. The way i calculate it now is by: q =...

02 December 2020 306 2 View

How can I determine the mass of a refrigerant (for example R744) in a evaporator when the refrigeration system is not working?

By knowing the specific volume on the inlet and on the outlet, is it correct to assume an average of those two specific volumes?

30 November 2020 3,807 3 View

Potassium formate/water or potassium formate+LiBr/Water are not available in the commercial sector for absorption refrigeration systems?

Although there are already studies in the literature but not much , the use of potassium formate/water or potassium formate+LiBr/Water are not available in the commercial sector for absorption...

16 November 2020 2,917 2 View

Is there any way to revive strains after a year stored in 4 degree refrigerator?

The strain is Candida tropicalis. It has been stored in 4 degree refrigerator for a year. Now we want to see if it is possible to revive the strain and make it function again. I tried to culture...

10 November 2020 5,650 9 View

No title

When producing a recombinant protein, harvested E. coli cells are normally refrigerated for 12-24 hours before homogenization to ensure protein inactivation due to foaming does not occur....

16 October 2020 7,373 0 View

How to calculate the dimensions and frequency, etc. of a pulse tube for required cold/hot temperatures?

Require to know the methodology for calculating dimensions (Lenght, diameter), operating frequency, etc. of a simple pulse tube refrigerator. Reference to handbook or manuscript would also be helpful.

29 September 2020 2,191 2 View