Hi,
I have performed immunoprecipitation using H3K9me3 antibody. My PCR results are shown in an attachment 1) H3K9me3, 2) CTCF 5x10^6 cells, 3) CTCF 10^6 cells, 4) input, 5) non template control.
By getting bands for H3K9me3 and input I can conclude my immunoprecipitation and subsequent amplification has been successful but why am I getting multiple bands in stead of just one? Is this normal for H3K9me3 or is it likely to be unspecific binding or primer dimers?
Thanks in advance