Any idea how does the araC gets translated? after the Pc promoter and operator (in the reverse direction), the araC gene directly starts. I looked into papers, but i couldn't find the RBS sequence for araC, can you please help?
I am trying for the structure optimization via Gaussian16 DFT calculation, for a system consisting of C,H, N, O and Ce atoms. Where I am using B3LYP/ 6,311G(d,p) C, H, N and O and B3LYP/SDD for...
30 June 2024 9,979 4 View
What basis set/basis sets will be useful for DFT (using Gaussian16) of cerium ion adsorption?
24 June 2024 6,927 3 View
explain the calculations
16 April 2024 5,668 3 View
how to measure open circuit voltage using autolab 3 electrode system and using nova software and how open circuit voltage is related to overpotential measured for electrochemical OER
24 March 2024 2,674 0 View
Hi , I have peptide that has NH2 at its N terminal. I am unable to perform energy minimization using CHARMM 36 force field as it is giving me error nh2 not found in residue topology. How should I...
27 February 2024 1,307 0 View
in my last latest paper on earthquake related to Turkey-Syria earthquake , the doi number is types incorrect. How do I correct this?
26 February 2024 5,843 2 View
After purifying Laccase enzyme (Bacterial origin), I tried performing enzyme assay using ABTS which is a known substrate. Still I am unable to validate its activity using the said substrate....
21 January 2024 5,159 0 View
Hello everyone! I am new to IF imaging and I was wondering if I could get clarification on this protocol for whole organoid imaging. Below is the protocol outlined from the paper: Human bone...
15 December 2023 7,161 0 View
Hello ! I have recently concluded the data analysis of a study which looks at Attitude, Anxiety towards robots and Trust as predictors of intention to use robots. The Multiple Regression Analysis...
17 November 2023 5,722 3 View
How do I orthogonalize a time series variable? Which software and analysis can be used for orthogonalization?
01 November 2023 5,154 2 View
I am on gene work to express gene of interest in some Bacillus species. Thus far, i need to try some RBS in Vector which is optimized for bacillus subtilis. Before i design vector to express in...
11 July 2024 7,847 1 View
I designed primer to clone the target gene with RBS, his tag. I want to confirm below: 1. if Is it okay that extra sequence longer than target gene part?? 2. I designed as RBS located after his...
10 May 2024 7,557 2 View
I designed primer to clone the target gene with RBS, his tag. I want to confirm below: 1. if Is it okay that extra sequence longer than target gene part?? Targeted gene part is 18bp long But...
10 May 2024 3,106 4 View
Dear all, I am currently working on Acinetobacter baumannii and I wanted to look for the RBS or Shine Dalgano sequence of a gene for cloning. I have tried searching prediction online tools like...
01 April 2024 9,750 0 View
I would like to know what is the function of ara gene mutation in DH5 alpha E. coli. The mutation chart says only this mutation will block arabinose metabolism. But what will happen with the...
24 February 2024 8,791 1 View
Hi everyone, I was looking for some tools (preferably web-based tools) to predict genes organized in operons in various bacterial sequences I currently work on (plasmids, chromosoms, etc.). For...
19 February 2024 8,922 2 View
We want to conduct the sugar profile (Arabinose, Xylose, Galactose etc...) of a fermentable feedstock from different varieties. To reduce the degradation chances of this fermentable feedstock we...
10 January 2024 1,548 3 View
for heterologous expression in B.subtilis, I have used a triple promoter for expression of 2 genes in one vector , each gene has its own RBS, but there is only one triple promoter before gene 1,...
01 September 2023 8,579 11 View
hello, I am curious about the use of RBS designer from the website: https://salislab.net/software/ how is it possible to use it for designing new RBS to overexpress protein in e.coli? thank you...
27 July 2023 6,193 2 View
Hi everyone I inserted a restriction site (5 nucleotides) between RBS and the start codon (these 5 nucleotides are upstream of multiple cloning site). after confirming my cloning, we started...
22 July 2023 1,363 5 View