I would like to know what is the function of ara gene mutation in DH5 alpha E. coli.
The mutation chart says only this mutation will block arabinose metabolism.
But what will happen with the transformed plasmids if arabinose metabolism is blocked?
Michael J. Benedik
Nothing will happen and everything will be fine.
The arabinose operon carries genes to permit growth on the sugar arabinose, E. coli carries many catabolic operons to permit growth on various sugars. So unless you intend to grow your strain on minimal media with arabinose as the sole carbon source (and I doubt you have any reason to do so), then you can ignore the ara mutation. It was just in the background of the strain but is not present for any particularly useful reason.
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