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Questions related from Aditi Konar
Any idea how does the araC gets translated? after the Pc promoter and operator (in the reverse direction), the araC gene directly starts. I looked into papers, but i couldn't find the RBS sequence...
11 October 2024 304 0 View
I am trying to purify a chimeric protein (his-tagged) via affinity chromatography for which I am using 30mM binding buffer (couldn't purify using 40mM)..but each time I am getting a contamination...
01 March 2023 1,318 5 View
I used bl21de3 cells with IPTG induction for expressing a protein. I have used the same cells and inducer for other proteins also which I could successfully purify. However this time i noticed the...
25 October 2022 5,314 2 View
What is the highest concentration of iptg induction that I can give for extremely low yield proteins? Conventionally it's 0.5 mM, i also tried 1mM but how higher can I go?
03 February 2022 2,258 1 View
Please give me some idea regarding azo cmc standard curve, how to construct one? The one given in the megazyme manual is very confusing.
21 November 2021 703 1 View
