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I am trying to purify a chimeric protein (his-tagged) via affinity chromatography for which I am using 30mM binding buffer (couldn't purify using 40mM)..but each time I am getting a contamination...
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I used bl21de3 cells with IPTG induction for expressing a protein. I have used the same cells and inducer for other proteins also which I could successfully purify. However this time i noticed the...
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What is the highest concentration of iptg induction that I can give for extremely low yield proteins? Conventionally it's 0.5 mM, i also tried 1mM but how higher can I go?
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Please give me some idea regarding azo cmc standard curve, how to construct one? The one given in the megazyme manual is very confusing.
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