Is it crucial to use ATCC recomended medium to expand cell lines upon arrival?

03 April 2018 6,390 4 View

How can i get rid of antibiotic (Penicillin/Streptomycin) tolerant bacteria from a cancer cell line?

I was trying to expand a cell line provided from a colleague but i noticed more than normal cell death in the culture just after the cells where thawed and attached to a t25 flask. Since then, the...

31 December 2017 2,124 8 View

Is DHT really needed to determine the IC50 of antiandrogens on breast cancer cell lines?

31 December 2017 9,342 2 View

What to consider before starting to develop drug-resistant cancer cell line models?

Hi. I want to start experiments in a few months, but i want to cover every single detail before starting with such time consuming protocols. To give a little bit of context, i aim to develop...

11 December 2017 3,832 4 View

Which are some DHT alternatives for IC50 assays in breast cancer cell lines?

10 November 2017 4,592 0 View

Why would the color of the DMEM F12 medium seem to be diluted?

The normal color of the media after being prepared with water, sodium bicarbonate and FBS should be an intense red color (as far as i know), but this time the color of the media is still red but...

09 October 2017 3,197 0 View

R1881 or DHT for AR receptor stimulation?

09 October 2017 8,640 0 View

Effect of quiescent/senescent cells on formazan production in MTT assays?

I was wondering if quiescent cells will have enough metabolic activity to interfere with the interpretation of the results (% of growth inhibition), in other words, if the formazan production...

07 August 2017 9,875 0 View

Will these changes affect the behaviour of the MCF7 cell line in future passages?

07 August 2017 5,412 6 View

Colorimetric assay for determining cancer cell growth inhibition?

07 August 2017 6,745 4 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

No title

02 March 2021 3,060 3 View