Hi, I have fatty floating cells in culture medium and I need to simply/quickly pellet them without degrading cells nor removing themfrom the plate. Any idea ? thanks
Hi Guillaume
I think it would be useful if you can give more details about your culture system. Do you have a monolayer of adherent cells and also some in suspension? So like a co-culture? Or is it purely a suspension culture?
I was going to suggest you to remove the media in which the cells are in suspension and transfer it to eppendorfs so you can centrifuge them and obtain a pellet of your cells. i used to do that for multiple analysis (westerns, pcr, etc) and it works fine. 1500 RPM will not damage your cells.
But if you do not want to remove the cells from that plate, maybe you can centrifuge the whole plate if you have access to a plate centrifuge. However, if you want a pellet, the ideal scenario would be to culture them in U shaped P96 well plate, so when you centrifuge the plate, the cells will precipitate in the rounded bottom. If you have a flat bottom plate, they will precipitate as a monolayer rather than a pellet.
Hope this is useful!
Hi Guillaume, I agree with Noelia that you can't pellet cells that float on top of the media.
Removing them from adherent cells would be easy.
Hi, tanks for your answers. I do have a plate centrifuge and I need to perform analyses on cells without removing them from the plates (U bottom). I have only suspension ones, not adherent but as they're fat loaded I can not pellet them in culture medium. I was thinking about adding a solvant (which one?) to make them 'sink' and perform analyses.
Hi Guillaume, you could try Ficoll.
https://www.researchgate.net/topic/Adipocytes
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