Hello,
I am going to perform some saliva's RNA analysis. I found in the litterature several protocols to collect and prepare saliva for RNA/DNA analysis. For main of them we have to use kits and there are a bit long. I also saw one using an extraction buffer containingTris HCl EDTA SDS for a whole night at 56°C.
I would like to know if there is a quick and simple protocol to prepare saliva for RNA analysis.
Thanks for your help.
Select a viral kit specific for the virus that you are using for your experiment.
I think this paper could help you:
Preprint SalivaDirect: Simple and sensitive molecular diagnostic test...
Researchers have simplified their test by replacing nucleic acid extraction with a simple proteinase K and heat treatment step and then testing specimens with a dualplex quantitative reverse transcription PCR (RT-qPCR) assay. So, they do not require specialized reagents or equipment for nucleic acid extraction.
To do RNA extraction from saliva, I have used the Invitrogen™ PureLink™ Viral RNA/DNA Mini Kit . It is very straightforward and follows a simple RNA extraction by column.
Select a viral kit specific for the virus that you are using for your experiment.
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