I usually use automated or column based kits to extract DNA from whole blood (collected from infected animals) and use the DNA for PCR based detection of parasites. However, when there is a delay from the suppliers of the kits, I am forced to use manual methods to extract the DNA. I am trying to find quick and simple protocols to extract DNA from whole blood.

In my search, I came across this article (https://bitesizebio.com/30255/how-to-choose-your-method-for-whole-blood-dna-extraction/) which provided a principle for one-step lysis extraction, but am unable to indentify the components of the lysis buffer.

Any advice or suggestion with regard to this will be of great help!