We recently tried to bond -SH to magnetic-Au NPs (in citrate buffer) surface of Nanobead (250nm diameter).
The SH is at the end of a apatemer stucture (SH-apatmer-alexa488) The apatmer has 15 bases.
The SH- come with the format of -S-S- it was activated with TCEP solution (pH=7).
The activated -SH-apatmer-Beacon stucutre then mixed together with the magnetic-Au NPs and sonicate 30min + room temperature incubation (2 h). However, after the preparation, we concentrate the beads using a strong magnatic feid, we can see the aggregated beads under 20X, but just no GFP flouresent signal for these bead.
Our guess is either -S-S to -SH activation or -SH bonding to Au surface was falied.
Could anyone with this experise provide us some sugguestion?
Would citrate buffer that comes with magnetic-Au NPs block -SH bonding to Au surface?
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