Hi all,

I'm recently using interaction of Nickel NTA lipid and His-tag protein to make proteoliposomes by simply mix liposomes (DOPC:DOPS:DOGS-Ni-NTA = 3:2:30%) and His tagged proteins at 4 celcius degrees overnight. Under TEM, I have the protein densely coated around the liposomes. However, under HS-AFM, once I incubated and bursted lipid on mica in Ni2+ containing buffer, the proteins no longer stayed on lipid, instead, they bound on the edge of lipid patches.

Could someone provides me a hint on why this happens and how to solve it? Thank you.