I plan to use the Favorgen RNA extraction kit, which specifies RNase-free ethanol. Since this is not available in my lab, can I use regular laboratory ethanol instead? Would this compromise RNA yield or integrity?
What isself motivation?
17 December 2022 7,439 2 View
dear all I have run the SDSM model for the 5th IPCC report. has the 6th report data been released for this model yet? thank you in advance Best, Marziyeh
10 January 2022 6,432 1 View
I am currently working on Glioblastoma and I am using CBD (15mg/kg) and my solvent is DMSO. Can you please help me with drug preparing protocol? I have dissolved CBD in 1:3 mixture of DMSO and PBS...
19 June 2021 4,668 2 View
It sounds that shorter λ (more energy) makes the wave more powerful to go through a specific thickness of the material, but the weaker wave does it better. How are the interactions of the wave...
09 April 2021 1,532 8 View
I use carbon paper in my research work as substrate in hydrogen evolution reaction. My sample has good properties based on characterization, but I reach different results in different...
12 March 2020 4,135 3 View
I am planning to design Revers primer and add His-tag to it . His-tag sequence is CAT CAT CAT CAT CAT CAT (6 histidin). They are added to Revers primer but : Should they be added to Revers primer...
09 January 2020 468 3 View
How do tourism services affect the development of creative tourism? How do services and infrastructures contribute to sustainable development?
18 August 2018 1,679 6 View
Dear All I simulated and verified a linear accelerator with Gate (7.2). The program was divided in two parts for more calculation. In the second part of the program, Phase Space with root format...
17 March 2018 5,113 1 View
Suppose that we know the nitrogen and sulfur content of the fuel.
29 September 2017 2,189 4 View
I've did it several times with DMF and everything was OK...but with DMSO the layer is very bad: pale brown and like some stains not evenly spreading... I've tested (with DMSO) both single step and...
17 June 2017 4,344 5 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...
10 August 2024 8,539 5 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...
06 August 2024 807 3 View
I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...
06 August 2024 1,367 3 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
99% pure ethanol was used for maceration also alternate methods along with the temperature and time to concentrate the extract can be specified
31 July 2024 5,113 4 View
I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...
31 July 2024 225 2 View
I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...
30 July 2024 6,420 1 View