Question regarding Calibration Curve and Dilution Integrity??

27 April 2023 0 6K Report

I have made 5 point calibration curve of my standard - 200, 400, 600, 800 & 1000 ppm. My sample's peak area in the HPLC is falling below the AUC. Can I extrapolate the calibration curve or should I make the dilutions with lower concentration and again form the calibration curve??

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