How to extract polysaccharids from Aloe gel?

I 'm trying to extract polysacharides from Aloe leaf and tried ethanol percipitation, but after getting the pellet. I used the protocol from following reference following the steps, but I don't...

27 January 2021 7,049 5 View

How much the microbial exploration in oil and gas industry is applicable?

Microbial oil and gas prospecting (MPOG) I would grateful it if you could provide me with your invaluable comments and ideas on the following questions: 1- Do you consider MPOG as a promising...

15 December 2020 9,895 2 View

How to get permission for any content reproduced in a new submission?

I have been asked to get the required permission for the figures reproduced in my review paper from other publications. All the figures which are reproduced from other publications appropriately...

13 February 2020 4,970 8 View

Why AMOVA test is not active in Arlequin?

I entered the data to Arlequin and under Genetic structure, the AMOVA option is not active, is this related to input data? The other options are active and will run the analysis. I enclosed the file.

15 August 2019 4,564 4 View

How are silver nanowires dispersed to isopropanol?

I synthesised nano wires using Polyol method then centrifuged and I have dispersed them into ethanol But they did not disperse into isopropanol. How do I disperse them into isopropanol (IPA)?

23 July 2019 4,428 1 View

How to interpret Stacks output?

10 July 2019 5,938 2 View

Classification of validation methods?

Classification of validation methods such as sum of squares errors and ... validation methods such as sum of squares errors and ...

17 April 2019 3,122 3 View

How to characterize insecticide resistance mechanism?

I have a question about investigating insecticide ( Neonicoitnoids) resistance mechanism. We have populations of insects that are resistant and susceptible to insecticide, and we are interested...

16 August 2018 8,782 2 View

Do you think that exist relation between desertification and climate change?

Today, desertification is one of major environmental problems.

08 October 2017 9,600 12 View

What is the best method of intering different temperature conditions into different areas of a unit solid volume?

21 July 2016 7,328 1 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Why does intensity drops as concentration increases?

Is there any reason for decrease in fluorescence emission intensity of HSA,BSA when increase the concentration ?

28 February 2021 3,653 4 View

Antibody anti-IgM Bovin conjugated Biotin ?

Hi everybody, Can anyone please suggest me an antibody anti-IgM Bovin conjugated Biotin ? I would like to use it in LUMINEX with a streptavidin conjugated phycoerythrin reporter. Thanks in...

25 February 2021 9,659 2 View

I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future ?

I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future without necessarily conducting the assay for an extended...

23 February 2021 7,842 1 View

What is the standard protocol in doing replicates for C. elegans lifespan assay on solid media (thru worm picking)?

I would like to know what is the standard protocol in the number of worms per plate and number of plates per concentration of extract X in a lifespan assay done in solid media and not automated...

23 February 2021 9,933 1 View

Why anti-CD20 or other antibodies are not used to label target cells in flow cytometry t-cell cytotoxicity assay?

New to the field of cell therapy. Why fluorescent-labeled antibodies to target cell antigens are not used for determining target cell count by flow cytometry in cytotoxicity assay (eg car-t)? Cell...

21 February 2021 3,278 3 View

Can anyone help or comment on solubilising chlorobutanol in aqueous injections in minimum time possible?

Please suggest solubilisation of chlorobutanol in injections. Tried pH and high temperature. But the issue is of assay drop. If anyone can suggest any solution. Its a commonly used excipient for...

21 February 2021 7,397 1 View