In D. salina in which beta carotene is accumulated throughout the cells, staining with iodine reagent will cause the whole cell to be stained.
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Here is a paper I recently read:
Salinity impairs photosynthetic capacity and enhances carotenoid-related gene expression and biosynthesis in tomato (Solanum lycopersicum L. cv. Micro-Tom). PeerJ, 8, e9742. https://doi.org/10.7717/peerj.9742
And one more:
Article ROS Induce β-Carotene Biosynthesis Caused by Changes of Phot...
Thank you so much for your answer.
The Dunaliella salina cells that I collected from natural habitats with high salinity(more than 30%), contained high amounts of starch and beta-carotene. I think, to cope with salinity, starting substance should be accumulated inside the cells to be used for the synthesis of protective substances such as beta carotene and glycerol. Probably, at the beginning of salt stress the gene expression for starch synthesis should be high. During salt stress, the stored starch will be hydrolyzed to glucose to be used for beta-carotene and glycerol synthesis.
Is it correct?
Iodine reagent helps fix or preserve the microalgae for subsequent analyzes in the laboratory.
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