I'd like to develop a quantitative mouse histamine ELISA instead of purchasing the commercial kits. Does anyone have any leads on a procedure that they use?
I will be culturing a cell line that requires glutathione. Should I use the reduced or oxidized version in my media? FYI, the basal medium is Leibovitz's L-15 medium and culture is performed at...
11 December 2019 8,336 3 View
It looks like anti-tryptase antibodies are available that cross-react with monkey but I'm unable to find monkey tryptase for use as a standard in an ELISA. The ELISA kits are insanely expensive.
01 February 2018 6,817 0 View
Are there any fluorescently labeled anti-Alpaca secondary antibodies raised in Donkey? So far I have only been able to find anti-Alpaca secondaries raised in Goat. Or is this not possible due to...
04 August 2024 4,255 1 View
Hi, I'm curious to know if data on chemical compounds from PubChem, such as water solubility properties, can be used to train a machine learning model for commercial purposes. Will this infringe...
30 July 2024 8,707 1 View
I am developing an ELISA kit for rotavirus A (G1P8 and G1P6). Does anyone know the weight (ng) of these two strains? Or to help me find their weight?
29 July 2024 3,138 2 View
First, thanks for taking the time to read my puzzle 🥹! I am currently working on the methodology part of my dissertation, and I have chosen a questionnaire as the instrument for my quantitative...
27 July 2024 3,457 3 View
I have three linkage maps that needs to be integrated to create a consensus map for QTL mapping.
25 July 2024 6,505 2 View
The literature on Supply Chain Integration (SCI), Supply Chain Management (SCM), and collaborations, spanning several decades, deserves special attention. This topic, although addressed for a long...
23 July 2024 5,672 3 View
Autonomous Language Learning can be implemented from primary to tertiary education. Practioners and students report on its effectiveness, however, there appears to be little quantitative or...
20 July 2024 2,592 2 View
Greetings! I am looking for materials on quantitative and qualitative research. What are the methods of collecting information and the principles of their implementation? I would like to make an...
20 July 2024 2,033 7 View
Dear Participants- Faculty and Researchers from Pakistani Higher Education Institutions, Thank you for participating in research study that aims to 'Analyze Individual and Institutional Factors...
18 July 2024 7,024 1 View
hi, I am facing difficulties in the développement of an ELISA sandwich. I have several experiences in developing ELISA but that’s the first time I can’t find any solution. My test is currently as...
16 July 2024 9,044 10 View