I will be culturing a cell line that requires glutathione. Should I use the reduced or oxidized version in my media? FYI, the basal medium is Leibovitz's L-15 medium and culture is performed at 37C without CO2.
Hi Eric
Glutathione protects cells from oxidative stress and contributes to a favorable redox environment both inside and outside the cell. It provides reducing equivalents to regenerate sulfhydryl compounds and antioxidants, and to convert peroxides to water or alcohols. Oxidized glutathione is rapidly reduced by glutathione reductase and under normal in vivo or in serum conditions very little exists as oxidized glutathione.
In vitro, glutathione generally exists in the presence of other thiol species. Because of its propensity to reduce these other molecules, it actually has a short half-life in the reduced form and must be continually reduced enzymatically. Glutathione is present in serum primarily in the reduced form. In the absence of serum or cells, it is likely to exist primarily in the oxidized form.
You have to use the reduced version. Probably your cells needy glutathione for reducing ROS.
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