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I will be culturing a cell line that requires glutathione. Should I use the reduced or oxidized version in my media? FYI, the basal medium is Leibovitz's L-15 medium and culture is performed at...
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I'd like to develop a quantitative mouse histamine ELISA instead of purchasing the commercial kits. Does anyone have any leads on a procedure that they use?
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It looks like anti-tryptase antibodies are available that cross-react with monkey but I'm unable to find monkey tryptase for use as a standard in an ELISA. The ELISA kits are insanely expensive.
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